Shoe shop Shoon, food store Oil and Vinegar, Collections and Past Times have all closed. All were situated in the shadow of Windsor Castle at the top of Peascod Street.
King Edward Court is also suffering in tough trading conditions and computer games store Game shut on Tuesday while a Sony outlet has also closed.
Business leaders are frantically working to make sure Windsor looks pristine before millions of visitors descend on the town. A hundred stores will take part in a spruce-up of shopfronts as part of the Windsor and Eton Facelift.
Windsor and Eton town centre manager, Paul Roach, said it was 'tough times' and just over nine per cent of the 350 units are empty.
He said: "I will be chasing up agents to see about any empty units as it is really important the town centre looks great this year and we have fully let shops units.
"Retailers are saying it is tough out there and challenging times for them and the main concern is how sustainable new stores are."
Mr Roach said there was some positive news as Superdry and Rolex stores are set to open in the coming months, while trade in lower Peascod Street was picking up.
King Edward Court manager Martin Miranda, who is also treasurer of Windsor District Chamber of Commerce said centre footfall is down five per cent and two out of the 35 retail units are empty.
He said: "Trade is not fantastic at the moment but we are confident all the units will be filled by the Olympics."
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